Grayfield Optical Inc - High Resolution Optical Microscopes

We are often asked about the maximum resolution capabilities of the Ergonom 4000 microscopes. In general, we quote the following:

Reflected Light: <100nm true resolution

Transmitted Light: 100nm true resolution

Note: Achievable resolution is dependant on the objectives used and the quality of the samples being observed. If the sample height is less than 50nm, achievable true resolution could be limited to 150nm.

For examples of this, please look at the page on Test Slides where we show images from two different certified test slides.

In practice, such high resolutions require samples of high quality to be able to fully utilize this capability. Please note that an important part of obtaining high resolution images is the type of recording media used.

As the OLS system works optically in real time and with natural colors, it is not necessary to wait for a sample to be scanned and converted into a computer image. Such high resolutions can be seen through the ocular where you will see a very clear precise image of the sample you are examining.

When very high resolutions need to be recorded, we recommend using photographic film as digital cameras have yet to achieve the resolutions film can offer. In general, it is the resolution of the photographic media which is the limiting factor on our microscopes. The optical system offers superb resolution and theoretically, even higher resolutions are possible (although at considerably more expense).

We use almost exclusively Nikon made objectives as we have found these to provide the highest quality of all the objectives we have tested.

Cancerous Cell being attacked by a T-Lymphocyte as seen through an Ergonom Microscope

With these distinctive features, the Ergonom microscopes close the yawning gap between conventional highest-quality optical microscopes and the scanning electron microscope (SEM).

The Ergonom technology assures faithful color reproduction of specimens/samples - a feature extremely important for detailed analysis. Even at high magnifications, observations of the living object are possible. Complete sequences can be recorded on video or film.

Specimens and samples are do not need any kind of staining or surface coating allowing observation in their natural state. This prevents damage and falsifications of the sample and allows for long term analysis of living specimens. The samples can be viewed at normal room temperature without any need for a vacuum. The specimens are not heated more than 5°C during observation under the microscope. Oil immersion is not needed with the Ergonom microscope systems.

Such features are ideal for long term observation of living specimens in their natural state without interfering with the specimens with stains, etc.

According to the physical laws of optics, the depth of field decreases with increasing magnification. The Ergonom microscope systems knows no relationship between depth of field, magnification, and resolution. The depth of field remains adjustable between "very large" and "zero" - a feature conferring unprecedented depth of field. Even the physical formula for the maximum resolution does not apply to the Ergonom microscopes. The true resolution can be significantly better than 250 nm.