Grayfield Optical Inc - High Resolution Optical Microscopes

We are often asked about the resolution capabilities of the Ergonom microscopes. In general, we quote the following:

Reflected Light: 100nm true resolution (if sample height is less than 50nm, 150nm true resolution possible)

Transmitted Light: <250nm true resolution (new model available with 300nm resolution)


For examples of this, please look at the page on Test Slides where we show images from two different certified test slides.

In practice, such high resolutions require samples of high quality to be able to fully utilize this capability. Please note that an important part of obtaining high resolution images is the type of recording media used.

As the OLS system works optically in real time and with natural colors, it is not necessary to wait for a sample to be scanned and converted into a computer image. Such high resolutions can be seen through the ocular where you will see a very clear precise image of the sample you are examining.

When very high resolutions need to be recorded, we recommend using photographic film as digital cameras have yet to achieve the resolutions film can offer. In general, it is the resolution of the photographic media which is the limiting factor on our microscopes. The optical system offers superb resolution and theoretically, even higher resolutions are possible (although at considerably more expense).

We use almost exclusively Nikon made objectives as we have found these to provide the highest quality of all the objectives we have tested.

Cancerous Cell being attacked by a T-Lymthocyte as seen through an Ergonom Microscope