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Historically, the diatom Amphipleura pellucida has often been used to test the resolution of microscopes. The holes have a diameter of just 180nm which is a challenge for all light microscopes to see. Here are some examples imaged through the microscope.

To see the images at full resolution, just click on them.

(Note: Magnifications given relate to a picture size of DIN A4).

6308x Holes cannot be seen (Click to enlarge)
Amphipleura pellucida: 6308x Holes cannot be seen clearly at this magnification
8705x Holes can now be seen (Click to enlarge)
Amphipleura pellucida: 8705x Holes can now be seen
Click to Enlarge
Click to enlarge
Click to enlarge
Diatom Triceratium favus (2020x)
Diatom Triceratium favus (2020x)
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