SeeNano in Comparison - Grayfield Optical Inc - High Resolution Optical Microscopes

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SeeNano in Comparison

Products
Ergonom 3000 microscope
Ergonom 4000 microscope
Features Compared

Conventional Optical

SeeNano Lab Series

SeeNano Pro Series

Microscopes

Lab-U

Lab-I

Lab-T

Pro-U

Pro-I

Pro-T

Resolution Incident

Theoretically, 200nm
Practically ~
400nm

n.a.

<200nm

 

<100nm

<100nm

 
Resolution Transmitted  

200nm

100nm

 

100nm

True magnification limit

800x (dry), 1600x (oil)

Up to 4500x (dry)

Greater than 8000x (dry)

Numerical Aperture

0.95 (dry), 1.40 (oil) N.A.

0.85 to 0.9 N.A. (dry)

0.85 to 0.95 N.A. (dry)

True color contrast

None at high mag.

Full, even at high mag.

Full, even at high mag.

White light source

Yes, for low res. only

Yes, even at max. res.

Yes, even at max. res.

Variable DOF

Low DOF, fixed

Low to extreme DOF

Low to extreme DOF

Contour sharpness

Poor at high mag.

High at all mag.

High at all mag.

Reflection free

No

Yes

Yes

Staining (etc.) required

Staining required

Staining not necessary

Staining not necessary

Oil immersion required

Yes, beyond 0.95 N.A.

No, max. 0.95 N.A. needed

No, 0.95 N.A./extended WD

Brightfield

Yes

Yes

Yes

Darkfield

Yes

Yes, but Grayfield better

Yes, but Grayfield better

Grayfield

No

Yes, with full color contrast

Yes, with full color contrast

Phase Contrast

Yes

Improved, with contour sharpness

Improved, with contour sharpness

Living samples in vitro

Yes, for limited time

Yes, for extended periods

Yes, for extended periods
when used with our chambers

Sample warmth level

Up to 60° C over room temp.

Up to 3° C over room temp.

Up to 3° C over room temp.

Transparent samples

Not possible

Yes, incl. absolute transp.

Yes, incl. absolute transp.

Notes:

  • Resolution values given for the SeeNano microscopes relate to the practical achievable resolution. The maximum theoretical (although not achievable) resolution is 10nm with the Grayfield Lens System.


  • The standard methods of measuring magnification in microscopes becomes too inaccurate beyond 1500x. We therefore determine the precise magnification factor by imaging a calibrated test slide with nanometer scale and printing that image on 10x15cm paper. The enlargement factor in relation to the grid is then used to calculate the actual magnification factor. Every objective combination is tested so that we can provide exact magnification ratios. As there are no parallax errors with our systems, it is possible to measure distance in any direction.


  • For each microscope, there are a wide range of dry objectives available to match your application, e.g. 2,5x, 4x, 10x, 20x, 40x, 60x, 80x, 100x with n.A. up to 0.95 - some with extended working distance (WD). As the costs of the individual objectives vary widely according to their specifications, general price quotes are not meaningful before your exact requirements have been determined.


  • The SeeNano Lab series, has been optimized to make it an affordable alternative to those considering high quality conventional optical microscope systems, yet with an image quality, depth of field and color contrast that is considerably better and especially suitable for live blood and live cell analysis. For technical reasons, it is not feasible to provide a SeeNano Lab microscope with both incident and tranmitted light in one unit (costs would be higher than two individual microscopes).


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